diff --git a/src/dawn_native/d3d12/RenderPipelineD3D12.cpp b/src/dawn_native/d3d12/RenderPipelineD3D12.cpp
index fc0331eac6..4190164a35 100644
--- a/src/dawn_native/d3d12/RenderPipelineD3D12.cpp
+++ b/src/dawn_native/d3d12/RenderPipelineD3D12.cpp
@@ -389,7 +389,7 @@ namespace dawn_native { namespace d3d12 {
         descriptorD3D12.DepthStencilState =
             ComputeDepthStencilDesc(GetDepthStencilStateDescriptor());
 
-        descriptorD3D12.SampleMask = UINT_MAX;
+        descriptorD3D12.SampleMask = GetSampleMask();
         descriptorD3D12.PrimitiveTopologyType = D3D12PrimitiveTopologyType(GetPrimitiveTopology());
         descriptorD3D12.SampleDesc.Count = GetSampleCount();
         descriptorD3D12.SampleDesc.Quality = 0;
diff --git a/src/dawn_native/opengl/BackendGL.cpp b/src/dawn_native/opengl/BackendGL.cpp
index 5ce73d88b5..2d3685980e 100644
--- a/src/dawn_native/opengl/BackendGL.cpp
+++ b/src/dawn_native/opengl/BackendGL.cpp
@@ -162,6 +162,7 @@ namespace dawn_native { namespace opengl {
             mFunctions.Enable(GL_PRIMITIVE_RESTART_FIXED_INDEX);
             mFunctions.Enable(GL_MULTISAMPLE);
             mFunctions.Enable(GL_FRAMEBUFFER_SRGB);
+            mFunctions.Enable(GL_SAMPLE_MASK);
 
             mPCIInfo.name = reinterpret_cast<const char*>(mFunctions.GetString(GL_RENDERER));
 
diff --git a/src/dawn_native/opengl/RenderPipelineGL.cpp b/src/dawn_native/opengl/RenderPipelineGL.cpp
index b16783ce90..441df88f8c 100644
--- a/src/dawn_native/opengl/RenderPipelineGL.cpp
+++ b/src/dawn_native/opengl/RenderPipelineGL.cpp
@@ -257,6 +257,8 @@ namespace dawn_native { namespace opengl {
 
         ApplyDepthStencilState(gl, GetDepthStencilStateDescriptor(), &persistentPipelineState);
 
+        gl.SampleMaski(0, GetSampleMask());
+
         for (uint32_t attachmentSlot : IterateBitSet(GetColorAttachmentsMask())) {
             ApplyColorState(gl, attachmentSlot, GetColorStateDescriptor(attachmentSlot));
         }
diff --git a/src/tests/end2end/MultisampledRenderingTests.cpp b/src/tests/end2end/MultisampledRenderingTests.cpp
index 4e3edfd3fe..3c17acdad9 100644
--- a/src/tests/end2end/MultisampledRenderingTests.cpp
+++ b/src/tests/end2end/MultisampledRenderingTests.cpp
@@ -525,9 +525,8 @@ TEST_P(MultisampledRenderingTest, ResolveInto2DArrayTexture) {
 // Test using one multisampled color attachment with resolve target can render correctly
 // with a non-default sample mask.
 TEST_P(MultisampledRenderingTest, ResolveInto2DTextureWithSampleMask) {
-    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in those
-    // backends.
-    DAWN_SKIP_TEST_IF(IsOpenGL() || IsMetal() || IsD3D12());
+    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in Metal.
+    DAWN_SKIP_TEST_IF(IsMetal());
 
     constexpr bool kTestDepth = false;
     // The second and third samples are included,
@@ -559,9 +558,8 @@ TEST_P(MultisampledRenderingTest, ResolveInto2DTextureWithSampleMask) {
 // Test using one multisampled color attachment with resolve target can render correctly
 // with the final sample mask empty.
 TEST_P(MultisampledRenderingTest, ResolveInto2DTextureWithEmptyFinalSampleMask) {
-    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in those
-    // backends.
-    DAWN_SKIP_TEST_IF(IsOpenGL() || IsMetal() || IsD3D12());
+    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in Metal.
+    DAWN_SKIP_TEST_IF(IsMetal());
 
     constexpr bool kTestDepth = false;
     // The third and fourth samples are included,
@@ -593,9 +591,8 @@ TEST_P(MultisampledRenderingTest, ResolveInto2DTextureWithEmptyFinalSampleMask)
 // Test doing MSAA resolve into multiple resolve targets works correctly with a non-default sample
 // mask.
 TEST_P(MultisampledRenderingTest, ResolveIntoMultipleResolveTargetsWithSampleMask) {
-    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in those
-    // backends.
-    DAWN_SKIP_TEST_IF(IsOpenGL() || IsMetal() || IsD3D12());
+    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in Metal.
+    DAWN_SKIP_TEST_IF(IsMetal());
 
     wgpu::TextureView multisampledColorView2 =
         CreateTextureForOutputAttachment(kColorFormat, kSampleCount).CreateView();
@@ -635,9 +632,8 @@ TEST_P(MultisampledRenderingTest, ResolveIntoMultipleResolveTargetsWithSampleMas
 
 // Test multisampled rendering with depth test works correctly with a non-default sample mask.
 TEST_P(MultisampledRenderingTest, MultisampledRenderingWithDepthTestAndSampleMask) {
-    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in those
-    // backends.
-    DAWN_SKIP_TEST_IF(IsOpenGL() || IsMetal() || IsD3D12());
+    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in Metal.
+    DAWN_SKIP_TEST_IF(IsMetal());
 
     // TODO(dawn:491): Find out why this test doesn't work on Windows Intel Vulkan.
     DAWN_SKIP_TEST_IF(IsWindows() && IsIntel() && IsVulkan());
@@ -702,9 +698,11 @@ TEST_P(MultisampledRenderingTest, MultisampledRenderingWithDepthTestAndSampleMas
 // Test using one multisampled color attachment with resolve target can render correctly
 // with non-default sample mask and shader-output mask.
 TEST_P(MultisampledRenderingTest, ResolveInto2DTextureWithSampleMaskAndShaderOutputMask) {
-    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in those
-    // backends.
-    DAWN_SKIP_TEST_IF(IsOpenGL() || IsMetal() || IsD3D12());
+    // TODO(dawn:491): Remove this condition after enabling sampleMask usage in Metal.
+    DAWN_SKIP_TEST_IF(IsMetal());
+
+    // TODO(dawn:491): Remove this when SPIRV-cross adds support for SV_Coverage in HLSL.
+    DAWN_SKIP_TEST_IF(IsD3D12());
 
     constexpr bool kTestDepth = false;
     wgpu::CommandEncoder commandEncoder = device.CreateCommandEncoder();